Journal: International Journal of Pharmaceutics: X

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

doi: 10.1016/j.ijpx.2026.100487

Figure Lengend Snippet: Neutralization of merozoites. A Purification of infected erythrocytes using the Percoll separation solution, displaying infected (black arrow) and normal (red arrow) erythrocytes. B Confocal fluorescent images of merozoites (blue), PECm-Allicin@LM (red), and their colocalization (purple). Scale bar = 50 μm. C Representative scatter plots of Hoechst 33342/CFDA-SE for the invasion test of merozoites and normal erythrocytes after drug treatment. D Intrusion rate (the percentage of cells in the Q2 area in each group relative to that in the Q2 area in the Model group). Data are presented as mean ± SEM ( n = 3), * P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The red fluorescent cell membrane dye DiD was purchased from Shanghai Titan Scientific Co., Ltd. (China).

Techniques: Neutralization, Purification, Infection

Journal: Human Mutation

Article Title: PLSCR3 Deficiency Triggers mtDNA‐Driven cGAS‐STING Activation to Potentiate Antitumor Immunity in Colorectal Cancer

doi: 10.1155/humu/8545428

Figure Lengend Snippet: PLSCR3 deficiency disrupts mitochondrial homeostasis in CRC cells. (A) Western blotting verified the reduction of PLSCR3 protein levels in HT29 cells. (B) RT‐qPCR analysis verified reduction of PLSCR3 mRNA levels normalized to GAPDH compared with negative control (NC) cells ( p < 0.0001, n = 3). (C) Representative JC‐1 staining (scale bar: 20 μ m) images showing red fluorescence (polarized mitochondria) and green fluorescence (depolarized mitochondria). (D) Quantification of the JC‐1 red/green fluorescence intensity ratio. Fluorescence intensity was quantified using ImageJ software as described in the section. (E) Real‐time oxygen consumption rate (OCR) profiles under sequential treatment with oligomycin, FCCP, and rotenone/Antimycin A. (F) Quantification of cytosolic mtDNA by qPCR using the D‐loop ratio in PLSCR3‐knockdown and control cells. Data are presented as mean ± SD from three independent experiments. Statistical significance was determined using an unpaired two‐tailed Student′s t ‐test for two‐group comparisons.

Article Snippet: Cells were incubated with JC‐1 dye (MedChemExpress, Cat. No. HY‐K0601) at 37°C in the dark, enabling accumulation in mitochondria; high membrane potential promoted J‐aggregate formation (red fluorescence), while depolarized mitochondria retained JC‐1 monomers (green fluorescence).

Techniques: Western Blot, Quantitative RT-PCR, Negative Control, Staining, Fluorescence, Software, Knockdown, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Estrogen-related receptor gamma is required for normal auditory innervation and is essential for hearing

doi: 10.64898/2026.05.02.722410

Figure Lengend Snippet: Maximum intensity projections of confocal z-stacks of cochlear whole mount preparations from Esrrg +/+ (A,C,E and G) and Esrrg fl/fl ;Sox10- Cre (B,D,F and H) mice immunolabelled with antibodies to Myosin7a (Blue) and NF-H (Green), co-stained with the cell plasma membrane dye CellMask™ orange (Magenta) to reveal the myelinated fibres. Esrrg fl/fl ;Sox10- Cre mice display defective myelination from early development (H) with reduced auditory fibres apparent at P1 (F) . (I) Abundance of myelinated fibres extracted from the fluorescent intensity of the CellMask™ orange signal at 8kHz and 24kHz from a 50µm by 59µm ROI in the OSL distal to the HP. ROI were acquired from 20x maximum intensity projections as shown in A, B. Data was acquired from 2-3 mice per genotype per sex. Maximum intensity projections of confocal z-stacks of apical coil cochlear cryosections from adult Esrrg +/+ (J) and Esrrg fl/fl ;Sox10- Cre (K) mice immunolabelled with antibodies to the heminodal proteins - Caspr (Magenta) and AnkG (Green). Maximum intensity projections of confocal z-stacks of cochlear whole mount preparations from adult Esrrg +/+ (L) and Esrrg fl/fl ;Sox10- Cre (M) mice immunolabelled with antibodies to the efferent innervation marker ChAT (green) and Myo7a (Magenta). Maximum intensity projections of confocal z-stacks of mid-coil cochlear cryosections from adult Esrrg +/+ (N) and Esrrg fl/fl ;Sox10- Cre (O) mice immunolabelled with antibodies to the pan-neuronal SGN marker βIII-Tubulin (Green) and the Type II marker Peripherin (Magenta). Note, Rosenthal’s canal (dashed lines) is smaller in Esrrg fl/fl ;Sox10- Cre mice (O) . (P-R) SGN density at P29 in Esrrg fl/fl ;Sox10- Cre mice compared to controls across basal, mid, and apical cochlear coils: (P) Type I & II, (Q) Type I and (R) Type II SGNs. Data was pooled from both male and female mice and acquired from 3 mice per genotype per sex. Data is plotted as mean values ± SD; *p<0.05; **p<0.01; ***p<0.001 unpaired t-test. Images represent data from 2-3 mice per genotype per sex. Scale bar: A,B: 20μm; C,D:100 μm; E,F: 20μm; G,H: 10μm; J,K: 10 μm; L,M: 10μm; N,O: 20 μm.

Article Snippet: In some experiments, 10nM Phalloidin-Atto 647N (Sigma-Aldrich, Gillingham, U.K.) was added to the secondary antibody solution to label f- actin for visualisation of hair cell stereocilia or CellMaskTM Orange (Invitrogen, #C10045) a fluorescent plasma membrane dye, diluted 1:5000 in 0.3% Triton-X 100 with PBS, was added as an extra 5-minute room temperature incubation after the secondary antibody step to visualise the myelin sheaths.

Techniques: Staining, Clinical Proteomics, Membrane, Marker

Journal: Infection and Immunity

Article Title: Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1

doi: 10.1128/iai.00523-25

Figure Lengend Snippet: Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

Article Snippet: CellBrite Green stain was purchased from Biotium (30021).

Techniques: Incubation, Staining, Confocal Microscopy, Fluorescence, Isolation